In accordance with the mandate implied in the preamble of the 1945 Constitution and Banyumas people’s insistence on the need of the higher Education, Banyumas formal and informal leaders initiated the need for establishing a University in Banyumas region. As a follow-up to this idea, it was formed Jenderal Soedirman University Trustees Foundation with notary Deed No.33 September 20th, 1961. Furthermore, because of the urgingof the people, officials, and National Army Force, Jenderal Soedirman University Trustees Foundation tried to realize the establishment of the University. By Decree of the President of the Republic of Indonesia No.195 September 23rd, 1963, Jenderal Soedirman University (UNSOED) was officially established, and was inaugurated by the Minister of PTIP Prof. Dr. Tojib Hadiwidjaja located at the Official Residence of Banyumas.

 UNSOED had three faculties at first, namely the Faculty of Agriculture, the Faculty of Biology, and the Faculty of Economics. In its development, UNSOED opened several more faculties, namely the Faculty of Animal Husbandry (1966), the Faculty of Law (1982), the Faculty of Social Sciences and Political Sciences (1993), the Postgraduate Program (1994). In 2007, Faculty of Medicine and Health Sciences as well as Faculty of Science and Engineering were established.

 In 2014, there was an organizational change in which the Faculty of Medicine and Health Sciences were developed into the Faculty of Medicine and Faculty of Health Sciences. In addition, the Faculty of Science and Technology was also developed into the Faculty of Engineering, Faculty of Mathematics, and Natural Sciences as well as Faculty of Fishesries and Marine Science. Still in the same year, UNSOED also opened Faculty of Humanities which was previously under the administration of the Faculty of Social and Political Science.

 Now, at the age of 53 years, UNSOED affirms its commitment as a University that is recognized worldwide as a center for rural resource developmnent and local wisdom. UNSOED currently also has in progress to develop strategic partnerships with fellow Universities, business people, the community and the central or regional goverment to jointly develop the potential that exists inorder to improve its role and actualization, so that Tri Dharma can be achieved and felt its benefits for the nation and state and human values.